Search results for "12s rrna"
showing 6 items of 6 documents
Integrative taxonomy demonstrates the unexpected synonymy between two predatory mite species: Cydnodromus idaeus and C. picanus (Acari: Phytoseiidae)
2011
Contact: tixier@supagro.inra.fr; Species of the mite family Phytoseiidae are well known as predators of mite pests all over the world. Their identification is thus of great interest for biological control. The specimens examined in this study belong to the species Cydnodromus idaeus (described from Brazil) and C. picanus (described from Chile). They were collected together on the same plant in Argentina. These species are mainly differentiated by the presence/absence of the dorsal solenostomes (gland openings) gd2. Some morphometric differences were observed between the Argentinian specimens and the type material of C. idaeus and C. picanus; however, they were not sufficient to support a sp…
The concept of genus within the family Phytoseiidae (Acari: Parasitiformes): historical review and phylogenetic analyses of the genus Neoseiulus Hugh…
2012
Systematic studies on the family Phytoseiidae were first conducted at the beginning of the 20th century but increased greatly after the Second World War. Various classifications have been proposed based on different characters such as: dorsal, ventral, and leg chaetotaxy; the shape of ventrianal and sternal shields; the shape of the insemination apparatus (spermatheca) and spermatodactylus; the number of teeth on the movable digit of chelicera; and dorsal and ventral adenotaxy. The genus concepts developed over the last five decades can be divided into two main categories or hypotheses. The first, supported mainly by Chant and McMurtry, focuses on dorsal and ventral chaetotaxy, and the gene…
12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products
2016
Abstract For a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairy products, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheeses of Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovis aries ) mitochondrial species-specific gene fragment. The use of species-specific primers for Bos taurus , Capra hircus and Ovis aries species, after electrophoresis on agarose gel, yielded fragments of 256 bp, 326 bp and 172 bp, respectively. Amplification by duplex - PCR of DNA pools from two species showed detection thresholds of 0.1% of “contaminant” D…
New contribution to the systematic status of various Mediterranean scorpionfish, as inferred from a mitochondrial DNA sequence.
2014
This study investigated the molecular phylogeny of 6 Mediterranean species of scorpionfish, belonging to the Scorpaenidae and Sebastidae family. Neighbor-Joining and Maximum Parsimony phylogenetic analyse, based on 424 base pairs of partial mitochondrial DNA sequences of the 12S-rRNA gene, revealed 2 main clades. One clade is represented by the Scorpaena genera (with the species S. notata, S. porcus, and S. scrofa) and another clade consists of the genera Helicolenus, Pterois, and Scorpaenodes. The molecular phylogeny showed that the Scorpaenodes genus (sub-family Scorpaeninae) is found within the clade of the species belonging to the other two sub-families (Pteroninae and Sebastinae). This…
Mites of the genus Typhlodromus (Acari: Phytoseiidae) from Southern France: combined morphological and molecular approaches for species identification
2019
Mites of the family Phytoseiidae are important predators for biological control applications. They occur naturally in ecosystems but their overall distribution is not completely known. This study presents results of surveys carried out in the south of France. It proposes the use of a combination of morphological and molecular approaches for species diagnosis. Eighteen species of the genus Typhlodromus are reported from southern France, of which nine belong to Typhlodromus (Anthoseius) and nine to Typhlodromus (Typhlodromus). Eight of these species are new to the French fauna. The mitochondrial DNA CytB gene from 85 specimens (18 species) and the 12S rRNA gene from 30 specimens (9 species) w…
Molecular-genetic-based contribution to the taxonomy of theAcanthocyclops robustusgroup
2013
Long-standing taxonomic problems involving the Acanthocyclops robustus–vernalis complex of freshwater cyclopoids have not been resolved. After Kiefer's designation of A. robustus Sars as an older synonym of Acanthocycops americanus Marsh, a lot of data indicating their differentiation have been accumulated. To handle this taxonomical problem, representative populations from type localities of the respective taxa and from other European and US sites were analyzed morphologically and genetically using mitochondrial COI and 12S rRNA. Molecular-genetic analysis revealed that the three species described at the end of the nineteenth century: A. robustus, A. americanus and A. vernalis are well-sep…